Professor Andy Sewell - Publications

Recent High Impact Publications

Bulek, A.M, D.K. Cole, A. Skowera, G. Dolton, S. Gras, F. Madura, A. Fuller, J.J. Miles, E. Gostick, D.A. Price, J.W. Drijfhout, R.R. Knight, N. Lissin, P.E. Molloy, L. Wooldridge, B.K. Jakobsen, J. Rossjohn, M. Peakman, P.J. Rizkallah and A.K. Sewell (2012) Structural basis of human beta-cell killing by preproinsulin-specific CD8+ T-cells in type 1 diabetes. Nature Immunology (published on line January 15th, 2012). This paper is set for print publication in the March issue and will be the subject of a News & Views article published on February 16th. Download

Nature Immunology (IMPACT FACTOR 25.7)

Varela-Rohena, A., P.E. Molloy, S. M. Dunn, Y. Li, M. M. Suhoski, R.G. Carroll, A. Milicic, T. Mahon, D.H. Sutton, B.E. Laugel, R. Moysey, B.J. Cameron, A. Vuidepot, M.E. Purbhoo, D.K. Cole, R.E. Phillips, C.H. June, B.K. Jakobsen, J.L. Riley* and A.K. Sewell* (2008) Control of HIV-1 immune escape by CD8 T-cells expressing enhanced T-cell receptor (*equal contribution). Download

Nature Medicine, 14, 1390-5. (IMPACT FACTOR 29)

Borbulevych O.Y., K.H. Piepenbrink, B.E. Gloor, D.R. Scott, R.F. Sommese D.K. Cole, A.K. Sewell, and B.M. Baker (2009) T cell receptor crossreactivity directed by antigen-dependent tuning of peptide-MHC molecular flexibility. Download

Immunity, 31, 885-96. (IMPACT FACTOR 24.2)

Skowera, A., R.J. Elps, R. Varela-Calvino, S. Arif, G.C. Huang, C. Van-Krinks, A. Zaremba, C. Rackham, J.S. Allen, T.I. Tree, M. Zhao, M. C.M. Dayan, A.K. Sewell, W. Unger, J.W. Drijfhout, F. Ossendorp, B.O. Roep and M. Peakman (2008) CTLs are targeted to kill beta cells in patients with type 1 diabetes through recognition of a glucose-regulated preproinsulin epitope. Download

Journal of Clinical Investigation, 118, 3390-3402. (IMPACT FACTOR 17.1)

Kasprowicz, V. S.M. Ward, A. Turner, A. Grammatikos, B. Nolan, L. Lewis-Ximenez, C. Sharp, J. Woodruff, V. Fleming, S. Sims, B.D. Walker, A. Sewell, G M. Lauer and P Klenerman (2008). Defining the directionalty and quality of CD8+ T cell heterologous immunity in hepatitis C virus infection. Download

Journal of Clinical Investigation, 118, 1143-53. (IMPACT FACTOR 17.1)

Jo, J., U Aichelle, N. Kersting, R. Klein, P. Aichele, E. Bisse, A. K Sewell, H.E. Blum, R. Bartenschlager, V. Lohmann and R. Thimme (2009) Analysis of CD8+ T-cell-mediated inhibition of hepatitis C virus replication using a novel immunological model. Download

Gastroenterology, 136, 1391-401. (IMPACT FACTOR 12.7)

Complete List (reverse chronology)

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2007-Present: Professor of Immunology, Cardiff University

115. Cole, D.K., Gallagher, K., Holland, C. S. Junaid, K. Wynn, E. Gostick, A.K. Sewell, A. Gallimore, K. Ladell, M-L. Gougeon, D.A. Price and A. Godkin (2012) Mutation of influenza virus haemagglutinin residues adjacent to a universal epitope selects high affinity CD4+ T cell receptors altering repertoire hierarchies. Nature Communications, DOI: 10.1038/ncomms1665.

114. Bulek, A.M, D.K. Cole, A. Skowera, G. Dolton, S. Gras, F. Madura, A. Fuller, J.J. Miles, E. Gostick, D.A. Price, J.W. Drijfhout, R.R. Knight, N. Lissin, P.E. Molloy, L. Wooldridge, B.K. Jakobsen, J. Rossjohn, M. Peakman, P.J. Rizkallah and A.K. Sewell (2012) Structural basis of human beta-cell killing by preproinsulin-specific CD8+ T-cells in type 1 diabetes. Nature Immunology (published on line January 15th, 2012). This paper is set for print publication in the March issue and will be the subject of a News & Views article published on February 16th. Download

113. Wooldridge, L., J. Ekeruche-Makinde, H.A. van den Berg, A. Skowera, J.J. Miles, M.P. Tan, G. Dolton, M. Clement, S. Llewellyn-Lacey, D.A. Price, M. Peakman and A.K. Sewell (2012) A single autoimmune T-cell receptor recognises over a million different peptides. Journal of Biological Chemistry, 287, 1168-77. Download

112. Bridgeman, J.S., A.K. Sewell, J.J. Miles, D.A. Price and D.K. Cole (2012) Structural and biophysical determinants of alpha beta T-cell antigen recognition, Immunology, 135, 1365-2567. Download

111. van den Berg, HA., C. Molina-Paris and A.K. Sewell (2011) Specific T-cell activation in an unspecific T-cell repertoire. Science Progress 94: 245-64.

110. Laugel, B., D.K. Cole, M. Clement, L. Wooldridge, D.A. Price and A.K. Sewell (2011). The multiple Roles of the CD8 co-receptor in T-cell biology: Opportunities for the design of targeted immunotherapies. Journal of Leukocyte Biology, 90, 1089-99.

109. Clement, M., K. Ladell, J. Ekeruche-Makinde, J.J. Miles, E.S.J. Edwards, G. Dolton, T. Williams, A.J.A. Schauenburg, D.K. Cole, S.N. Lauder, A.M. Gallimore, A.J. Godkin, S.R. Burrows, D.A. Price, L. Wooldridge* and A.K. Sewell* (2011) Anti-CD8 antibodies can trigger CD8+ T cell effector function in the absence of TCR engagement and improve peptide-MHCI tetramer staining. Journal of Immunology, 187, 654-63 (*Equal contribution). Download

108. Miles, K.M., J.J. Miles, F. Madura, D.K. Cole* and A.K. Sewell* (2010). Real time detection of peptide-MHC dissociation reveals that improvement of primary MHC-binding residues can have a minimal, or no, effect on stability. Molecular Immunology, 48, 728-32. (*Equal contribution). Download

107. Miles, J.J., A.M. Bulek, D.K. Cole, E. Gostick, A.J.A. Schauenburg, G. Dolton, V. Venturi, M.P. Davenport, M.P. Tan, S.R. Burrows, L. Wooldridge, D.A. Price, P.J. Rizkallah and A.K. Sewell (2010). Genetic and Structural Basis for Selection of a Ubiquitous T Cell Receptor Deployed in Epstein-Barr Virus Infection. PLoS Pathogens 6, e1001198. doi:10.1371/journal.ppat.1001198. Download

106. Tarbe M, I. Azcune, E. Balentová, J.J. Miles, E Edwards, K.M. Miles, P. Do, B.M. Baker, A.K. Sewell, J.M. Aizpurua, C. Douat-Casassus and S. Quideau (2010). Design, synthesis and evaluation of beta-lactam antigenic peptide hybrids; unusual opening of the beta-lactam ring in acidic media. Org. Biomol. Chem. 8, 5345-53. Download

105. Wooldridge, L., B. Laugel, J. Ekeruche, M. Clement, H van den Berg, D.A. Price and A.K. Sewell (2010) CD8 controls T cell crossreactivity. Journal of Immunology, 185, 4625-32. Download

104. Cole, D.K., E.S.J. Edwards, K.K. Wynn, M. Clement, J.J. Miles, K. Ladell, J. Ekeruche, K. Adams, A. Skowera, M. Peakman, L. Wooldridge, D.A. Price and A.K. Sewell (2010) Modification of MHC anchor residues generates heteroclitic peptides that alter TCR binding and T-cell recognition. Journal of Immunology, 185, 2600-10. Download

103. Schaubert, K.L., D.A. Price, J.R. Salkowitz, A.K. Sewell, J. Sidney, T.E. Asher, S.E. Blondelle, S. Adams, F.M. Marincola, A. Joseph, A. Sette, D.C. Douek, V. Ayyavoo, W. Storkus, M-Y. Leung, H.L. Ng, O.O. Yang, H. Goldstein, D.B. Wilson and J. Kan-Mitchell (2010). Generation of robust CD8+ T cell responses against subdominant epitopes in conserved regions of HIV-1 by repertoire mining with mimotopes. European Journal of Immunology, 40, 1950-1962. Download

102. Wooldridge L., M. Clement, A. Lissina, E.S. Edwards, K. Ladell, J. Ekeruche, R.E. Hewitt, B. Laugel, E. Gostick, D.K. Cole, R. Debets, C. Berrevoets, J.J. Miles, S.R. Burrows, D.A. Price and A.K. Sewell (2010). MHC class I molecules with Superenhanced CD8 binding properties bypass the requirement for cognate TCR recognition and nonspecifically activate CTLs. Journal of Immunology, 184, 3357-66. Download

101. Borbulevych, O.Y., K.H. Piepenbrink, B.E. Gloor, D.R. Scott, R.F. Sommese, D.K. Cole, A.K. Sewell and B.M. Baker (2009). T cell receptor cross-reactivity directed by antigen-dependent tuning of peptide-MHC molecular flexibility. Immunity, 31, 885-96. Download

100. Chen, J.L., A.J. Morgan, G. Stewart-Jones, D. Shepherd, G. Bossi, L. Wooldridge, S.L. Hutchinson, A.K. Sewell, G.M. Griffiths, P.A. van der Mewre, E.Y. Jones, A. Galione and V. Cerundolo (2010) Ca2+ Release from the Endoplasmic Reticulum of NY-ESO-1-Specific T Cells is Modulated by the affinity of TCR and by the use of CD8 coreceptor. Journal of Immunology, 184, 1829-39. Download

99. Walker, L.J, A.K. Sewell and P. Klenerman (2009) T cell sensitivity and the outcome of viral infection. Clinical and Experimental Immunology, 159, 245-55. Download

98. Horlock, C., B. Stott, J. Dyson, G. Ogg, T. McPherson, L. Jones, A.K. Sewell, L. Wooldridge, D.K. Cole, J. Stebbing and P. Savage (2009). ELISPOT and functional T cell analyses using HLA mono-specific target cells. Journal of Immunological Methods 350, 150-60. Download

97. Cole, D.K., F. Yuan, P.J Rizkallah, J.J. Miles, E. Gostick, D.A. Price, G.F Gao, B.K Jakobsen and A.K Sewell (2009). Germline-governed recognition of a cancer epitope by an immunodominat human T-cell receptor. Journal of Biological Chemistry 284, 27281-9. Download

96. Chouduri, K., M. Parker, D.K. Cole, A.M. Mipcic, M. Shaw, A.K. Sewell, T. Dong, K.G. Gould and P.A van der Merwe (2009) Peptide-MHC dimensions control proximal kinase-phosphate balance during T cell activation. Journal of Biological Chemistry, 284, 26096-105. Download

95. Varela-Rohena, A., B.K. Jakobsen, A.K. Sewell, C.H. June and J.L. Riley (2009) Are affinity-enhanced T cells the future of HIV therapy? HIV Therapy, 3, 105-8. Download

94. Jo, J., U Aichelle, N. Kersting, R. Klein, P. Aichele, E. Bisse, A. K Sewell, H.E. Blum, R. Bartenschlager, V. Lohmann and R. Thimme (2009) Analysis of CD8+ T-cell-mediated inhibition of hepatitis C virus replication using a novel immunological model. Gastroenterology, 136, 1391-401. Download

93. Wooldridge, L., A. Lissina, D.K. Cole, H. van den Berg, D.A. Price and A.K. Sewell (2008) Tricks with Tetramers: How to get the most from multimeric peptide-MHC. Immunology, 126, 147-64. Download

92. Lissina A., K. Ladell, A. Skowera, M. Clement, E. Edwards, R. Seggwiss, H. van den Berg, E. Gostick, K. Gallagher, E. Jones, J.J. Melenhorst, A.J. Godkin, M. Peakman, D.A. Price, L. Wooldridge* and A.K. Sewell* (2009) Protein kinase inhibitors substantially improve the physical detection of T-cells with peptide-MHC tetramers. Journal of Immunological Methods, 340, 11-24. Download

91. Varela-Rohena, A. P.E. Molloy, S. M. Dunn, Y. Li M. M. Suhoski, R.G. Carroll, A. Milicic, T. Mahon, D.H. Sutton, B.E. Laugel, R. Moysey, B.J. Cameron, A. Vuidepot, M.E. Purbhoo, D.K. Cole, R.E. Phillips, C.H. June, B.K. Jakobsen, J.L. Riley* and A.K. Sewell* (2008) Control of HIV-1 immune escape by CD8 T-cells expressing enhanced T-cell receptor Nature Medicine 14, 1390-5. (*equal contribution) Download

90. Skowera, A., R.J. Elps, R. Varela-Calvino, S. Arif, G.C. Huang, C. Van-Krinks, A. Zaremba, C. Rackham, J.S. Allen, T.I. Tree, M. Zhao, M. C.M. Dayan, A.K. Sewell, W. Unger, J.W. Drijfhout, F. Ossendorp, B.O. Roep and M. Peakman (2008) CTLs are targeted to kill beta cells in patients with type 1 diabetes through recognition of a glucose-regulated preproinsupn epitope. Journal of Clinical Investigation, 118, 3390-3402. Download

89. Melenhorst, J.J., P. Scheinberg, A. Lissina, P.K. Chattopadhyay, E. Gostick, D.K. Cole, L. Wooldridge, H.A. van den Berg, E. Bornstein, N.F. Hensel, D.C. Douek, M. Roederer, A.K. Sewell, A.J. Barret and D.A. Price (2008) Detection of low avidity CD8+ T cells with coreceptor-enhanced peptide-MHC class I tetramers. Journal of Immunological Methods, 338, 31-9. Download

88. Chattopadhyay, P.K., J.J. Melenhorst, K. Ladell, E. Gostick, P. Scheinberg, A. J Barrett, L. Wooldridge, M. Roederer, A.K. Sewell, D.A. Price (2008). Techniques to improve the direct ex vivo detection of low frequency antigen-specific CD8+ T cells with peptide-major histocompatibility complex class I tetramers. Cytometry 73, 1001-9. Download

87. Scriba, T.J., S. Sierro. E.L. Brown, R.E. Phillips, A.K. Sewell and R.C. Massey (2008) The S. aureus Eap protein activates expression of proinflammatory cytokines by human leukocytes. Infection and Immunity, 76, 2164-68. Download

86. Weichsel, R, C. Dix, L. Wooldridge, M. Clement, A. Fenton-May, A.K. Sewell, J. Zezula, E. Greine, E. Gostick, D.A. Price, H. Einsele and R. Seggewis (2008) Profound inhibition of antigen-specific T cell effector functions by dasatinib. Clinical Cancer Research, 14, 2484-91. Download

85. Kasprowicz, V. S.M. Ward, A. Turner, A. Grammatikos, B. Nolan, L. Lewis-Ximenez, C. Sharp, J. Woodruff, V. Fleming, S. Sims, B.D. Walker, A. Sewell, G M. Lauer and P Klenerman (2008). Defining the directionapty and quapty of CD8+ T cell heterologous immunity in hepatitis C virus infection. Journal of Clinical Investigation, 118, 1143-53. Download

84. Cole, D.K., S.M. Dunn, M. Sami, J.M. Boulter, A.K. Sewell and B.K. Jakobsen (2008) Co-engagement of the CD8α/α co-receptor to peptide-major histocompatiliipty complexes: The effect of T-cell receptor docking on CD8 binding. Molecular Immunology, 45, 2700-9. Download

83. Massey, R.C., T.J. Scriba, E.L. Brown, R.E. Philpps and A.K. Sewell (2007) The use of peptide-MHC tetramer technology to study interactions between S. aureus proteins (TSST-1 and Eap) and human cell. Infection and Immunity, 75, 5711-5. Download

82. Laugel, B., H.A. van den Berg, E. Gostick, D.K. Cole, L. Wooldridge, J. Boulter, A. Milicic, D.A. Price and A.K. Sewell (2007) Different T cell receptor affinity thresholds and CD8 coreceptor dependence govern cytotoxic T lymphocyte activation and tetramer binding properties. Journal of Biological Chemistry, 282, 23799-23810. Download

81. van den Berg, H., L. Wooldridge, B. Laugel and A.K. Sewell (2007) Coreceptor CD8-driven Modulation of T-Cell Antigen Specificity. Journal of Theoretical Biology 249, 395-408. Download

80. Yan, F., T. Georgiou, T. Hilton, E. Gostick, D.A. Price, A.K. Sewell, R. Moysey, J. Gavarret, A. Vuidepot, M. Sami, J.I. Bell, G.F. Gao, P.J. Rizkallah and B.K. Jakobsen (2007) Crystapzation and preliminary X-ray structural studies of a Melan A pMHC-TCR complex. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 63, 758-60. Download

79. M.A. Purbhoo, M. A., Y. p, D. H. Sutton, J. E. Brewer, E. Gostick, G. Bossi, B. Laugel, R. Moysey, E. Baston, N. Liddy, B. Cameron, A.D. Bennett, R. Ashfield, A. Mipcic, D.A. Price, B. J. Classon, A.K. Sewell and B.K. Jakobsen (2007) The A2- hTERT540-548 peptide is not detected on tumor cells by a CTL clone or a high affinity TCR. Molecular Cancer Therapeutics, 6, 2081-91. Download

78. Wooldridge, L., S.L. Hutchinson, A. Lissina, J. Vernazza, E. Gostick, B. Laugel, S. Tafuro, A. Oxenius, F. Mirza, P. R. Dunbar, J.M. Boulter, Meir Glick, V. Cerundolo, H. A. van den Berg, D. A. Price and A.K. Sewell (2007) Enhanced immunogenicity of CTL antigens through mutation of the CD8 binding MHC class I invariant region. European Journal of Immunology, 37, 1323-1333. Download

77. Schaubert, K., D.A. Price, N. Frahn, J. Li, H.L. Ng, A. Joseph, E. Paul, B. Majumder, V. Ayyavoo, E. Gostick, S. Adams, F. M. marincola, A.K. Sewell, M. Atfeld, J.M. Brenchley, D.C. Douek, O.O. yang, C. Brander, H. Goldstein and J. Kan-Mitchell (2007) Availabipty of a diversely avid CD8+ T cell repertoire specific for the subdominant HLA A2-restricted HIV-1 Gag p2419-27 epitope. Journal of Immunology, 178, 7756-66. Download

76. Laugel, B., D.A. Price, A. Milicic and A.K. Sewell (2007) CD8 exerts differential effects on the deployment of cytotoxic T lymphocyte effector functions. European Journal of Immunology, 37, 905-913. Download

75. Boulter, J.M., N Schmits, A.K. Sewell, A.J. Godkin, M.F. Bachmann and A.M. Galimore (2007) A very short half-pfe TCR-pMHC interaction can mediate a potent agonist T cell response. European Journal of Immunology, 37, 798-806. Download

74. Gostick, E., D.K. Cole, S. L. Hutchinson, L. Wooldridge, S. Tafuro, B. Laugel, A. Lissina, A. Oxenius, J.M. Boulter, D. A. Price and A. K. Sewell (2007) Functional and biophysical characterization of an HLA A*6801-restricted HIV-specific T cell receptor. European Journal of Immunology, 37, 479-86. Download

73. Cole, D.K., N.J. Pumphrey, J.M. Boulter, M. Sami, J.I. Bell, G. F. Gao, A.K. Sewell and Bent K. Jakobsen (2007) Human T-Cell Receptor Binding Affinity is Governed by Major Histocompatibipty Complex Class Restriction (2007). Journal of Immunology, 178, 5727-34. Download

72. Mitchell, M.S., T. A. Lund, A. K. Sewell, F. M. Marincola, E. Paul, K. Schroder, D. B. Wilson and J. Kan-Mitchell (2007) The cytotoxic T cell response to peptide analogs of the HLA-A*0201-restricted MUC1 signal sequence epitope, M1.2. Cancer Immunology and Immunotherapy, 56, 287-301. Download

2001-6 Wellcome Trust Senior Fellow, University of Oxford.

71. Fleming, V., Feil, E., A. K. Sewell, N. Day, A. Buckling and R. C. Massey (2006) Agr interference between clinical Staphylococcus aureus strains in an insect model of virulence Journal of Bacteriology, 188, 7686-8. Download

70. Wooldridge, L., T. J. Scriba, A. Milicic, E. Gostick, D. A. Price, R. E. Phillips and A. K. Sewell (2006). The choice of anti-CD8 or anti-CD4 antibody clone profoundly affects staining with peptide-MHC class I and class II tetramers European Journal of Immunology, 36, 1847-55. Download

69. B. Laugel, D.A. Price and A.K. Sewell (2006) TCR-directed therapeutics, Nature Biotechnology, 24, 615-6. Download

68. Scriba, T.J., M. Purbhoo, C. L. Day, N. Robinson, S. Fidler, J. Fox, J. N. Weber, P. Klenerman, A. K. Sewell and R.E. Phillips (2005). Ultra-sensitive detection and phenotyping of CD4+ T cells with optimized HLA class II tetramer staining Journal of Immunology, 175, 6334-43. Download

67. Rezvani, K, D.A Price, J. Brenchley, Y. Kipcal, E. Gostick, A.K. Sewell, G. Sconocchia, Roger Kurlander, D.C. Douek and John Barrett (2005). Transfer of defined T cell clones with graft-versus-leukemia (GVL) activity from donor to recipient in stem cell transplantation: identification, quantification and characterization. Clinical Cancer Research, 11, 8799-8807. Download

66. Price, D.A., J.M. Brenchley, L.E. Ruff, M. R. Betts, B.J. Hill, M. Roederer. R. Koup, D.R., S.A. Migueles, E. Gostick, L. Wooldridge, A.K. Sewell, M. Connors and D.C. Douek (2005) Avidity for antigen shapes clonal dominance in CD8+ T cell populations specific for persistant DNA viruses. Journal of Experimental Medicine, 202, 1349-61. Download

65. Milicic, A. D.A. Price, P. Zimbwa, B. L. Booth, H.L. Brown, P.J. Easterbrook, K. Olsen, N. Robinson, U. Gileadi, A.K. Sewell, V. Cerundolo, and R.E. Phillips (2005) CD8+ T cell flanking mutations disrupt proteasomal processing of HIV-1 Nef. Journal of Immunology, 175, 4618-4626. Download

64. Wooldridge, L., H. A. van den Berg, M. Glick, E. Gostick, J.M. Brenchley, D.C. Douek, D.A. Price and A.K. Sewell (2005). Interaction between the CD8 coreceptor and MHC class I stabilizes TCR-antigen complexes at the cell surface. Journal of Biological Chemistry, 280, 27491-27501. Download

63. Chen, J-L., G. Stewart-Jones, G. Bossi, N. M. Lissin, L. Wooldridge , E. Choi, G. Held, P.R. Dunbar, R. M. Esnouf, M. Sami, J. M. Boulter, P. Rizkallah, C. Renner, A.K. Sewell, P.A. van der Merwe, B.K. Jakobsen, G. Griffiths, E.Y. Jones and V. Cerundolo (2005) Structural and kinetic basis for heightened immunogenicity of T-cell vaccines Journal of Experimental Medicine, 201, 1243-55. Download

62. Leslie, A., D. Kavanagh, I. Honeyborne, K. Pfafferott, C. Edwards, T. Pillay, L. Hilton, C. Thobakgale, D. Ramduth, R. Draenert, S. Legall, G. Luzzi, A. Edwards, C. Brander, A.K. Sewell, S. Moore, J. Mullins, C. Moore, S. Mallal, N. Bhardwaj, K. Yusim, R. Phillips, P. Klenerman, B. Korber, P. Kiepiela, B. Walker and P. Goulder (2005) Transmission and accumulation of CTL escape variants explains apparent negative selection in HIV J. Exp. Med. 201, 891-902. Download

61. Molloy, P.E., A. K. Sewell and B. K. Jakobsen (2005) Soluble T cell receptors: novel immunotherapies. Current Opinion in Pharmacology, 5, 438-43. Download

60. Hewitt, R.E., A. Lissina,, A. E. Green, E. S. Slay, D. A. Price and A.K. Sewell (2005) The bisphosphonate acute phase response: Rapid and copious production of proinflammatory cytokines by peripheral blood γδ T cells in response to aminobisphosphonates is inhibited by statins Clinical and Experimental Immunology, 139, 101-111. Download

59. Laugel B., J.M. Boulter, N. Lissin, A. Vuidepot, Y. Li, Emma Gostick, L. E. Crotty, D.C. Douek, J. Hemelaar, D.A. Price, B.K. Jakobsen and A.K. Sewell (2005) Design of soluble recombinant T cell receptors for antigen targeting and T cell inhibition. Journal of Biological Chemistry, 280, 1882-1892. Download

58. Green, A. E., A. Lissina, S. L. Hutchinson, R. E. Hewitt, B. Temple, D. James, J. M. Boulter, D. A. Price and A. K. Sewell (2004) Recognition of non-peptide antigens by human Vγ9Vδ2 T cells requires contact with cells of human origin. Clinical and Experimental Immunology, 136, 472-82. Download

57. Gao B., A. Williams, Sewell A and T. Elliott (2004) Generation of a functional, soluble tapasin protein from an alternatively spliced mRNA. Genes Immun. 5, 101-8. Download

56. Wooldridge, L., S. L. Hutchinson, E. M. Choi, A. Lissina, E. Jones, F. Mirza, P. R. Dunbar, D. A. Price, V. Cerundolo, A. K. Sewell (2003) Anti-CD8 antibodies can inhibit or enhance peptide-MHC class I (pMHCI) multimer binding: This is paralleled by their effects on cytotoxic T lymphocyte activation and occurs in the absence of an interaction between pMHCI and CD8 on the cell surface. Journal of Immunology, 171, 6650-6660. Download

55. Choi, E. M-L., J-L. Chen, L. Wooldridge, M. Sapo, A. Lissina, N. Lissin, I. F. Hermans, J. D. Silk, F. Mirza, M. J. Palmowski, P. R. Dunbar, B. K. Jakobsen, A. K. Sewell and V. Cerundolo (2003) High avidity antigen specific CTL identified by CD8 independent tetramer staining. Journal of Immunology, 171, 5116-5123. Download

54. Green A, Hutchinson S, Boulter J, Lissina A, Temple B, Price D and Sewell A (2003). Activation of Vgamma9Vdelta2 T cells requires contact with a cell of human origin. Immunology, 110: 120-1.

53. Hutchinson, S. L. L. Wooldridge, S. Tafuro, B. Laugel, M. Glick, J. M. Boulter, B. K. Jakobsen, D. A. Price and A. K. Sewell (2003) The CD8 T cell coreceptor exhibits disproportionate biological activity at extremely low binding affinities. Journal of Biological Chemistry, 278, 24285-93. Download

52. Wooldridge, L. and A. Sewell (2002) HIV strategically targets HIV-specific T cells. Trends in Immunology, 23, 474. Download

51. Oxenius, A., A. K. Sewell, S. J. Dawson, H. F. Guenthard, G. M. Gillespie, C. Fagard, B. Hirschel, Rodney E. Phillips and David A. Price (2002) Functional discrepancies in HIV-specific CD8+ T lymphocyte populations are related to plasma virus load. Journal of Clinical Immunology, 22, 363-374. Download

50. Price, D. A and A. K. Sewell (2002) Quantitative clonotypic analysis of antigen-specific T-cell responses. Trends in Immunology, 23, 336-337. Download

49. Glick, M., D. A. Price, A-L. Vuidepot, T. B. Andersen, S. L. Hutchinson, A. K. Sewell, J. M. Boulter, A. Oxenius, P. R. Dunbar, V. Cerundolo, J. I. Bell W G. Richards and B. K. Jakobsen (2002) CD8+ T cell antagonists based on 2 microglobulin Journal of Biological Chemistry, 227, 20840-20846. Download

48. Sewell, A. K. (2002) Solving the mystery: CD8β delivers the TCR to lipid rafts. Trends in Immunology, 23, 124. Download

47. Kelleher, A. D., A. K. Sewell and D. A. Price (2002) Dyslipidaemia due to retroviral protease inhibitors. Nature Medicine, 8, 308-9. Download

46. Sewell, A. K. (2002) Breaking the kinetic window of T cell activation. Trends in Immunology, 23, 67. Download

45. Sewell, A. K., Bruce L. Booth Jr., Vincenzo Cerundolo, Rodney E. Phillips and David A. Price (2001). Differential processing of HLA A2-restricted HIV-1 cytotoxic T lymphocyte epitopes. Viral Immunology, 15, 193-196.

44. Sewell, A. K. and D. A. Price (2001). An inter-species difference in CD8. Trends in Immunology, 22, 595. Download

43. Purbhoo, M. A., J. Boulter, D. A. Price, C. S. Hourigan, P. R. Dunbar, K. Olsen, S. Dawson, B. K. Jakobsen, R. E. Phillips, J. I. Bell and A. K. Sewell (2001). The human CD8 coreceptor effects cytotoxic T cell activation and antigen sensitivity primarily by mediating complete phosphorylation of the T cell receptor zeta chain Journal of Biological Chemistry, 276, 32786-92. Download

42. Kellerher, A. D., B. L. Booth, A. K. Sewell, A. Oxenius, V. Cerundolo, A. J. McMichael, R. E. Phillips and D. A. Price (2001). The effects of retroviral protease inhibitors on proteasome function and processing of HIV-derived MHC class I-restricted cytotoxic T lymphocyte epitopes. AIDS Research and Human Retroviruses, 17, 1063-6.

41. Xu, XN, M. A. Purbhoo, N. Chen, J. Mongkolsapaya, J. H. Cox, U. Meier, S. Tafuro, P. R. Dunbar, A. K. Sewell, C. S. Hourigan, V. Appay, V. Cerundolo, A. J. McMichael and G. R. Screaton (2001). A novel approach to antigen-specific deletion of CTL with minimal cellular activation using alpha three domain mutants of MHC class I/peptide complexes. Immunity, 14, 591-602. Download

40. Oxenius, A., D. A. Price, S. J. Dawson, T. Tun, Phillips, R. E. and A. K. Sewell (2001). Cross-staining of cytotoxic T lymphocyte populations with peptide-MHC class I multimers of natural HIV-1 variant antigens. AIDS, 15, 121-123.

39. Sewell, A. K. and D. A. Price (2001). Dendritic cells and transmission of HIV-1. Trends in Immunology, 22, 173-175. Download

38. Hjorth-Sorensen, B., E. R. Hoffman, N. M. Lissin, A. K. Sewell and B. K. Jakobsen (2001) Activation of heat shock transcription factor in yeast is not influenced by the levels of expression of heat shock proteins. Molecular Microbiology, 39, 914-23. Download

1996-2001 Postdoc, University of Oxford

37. Price, D. A., A. Oxenius, P. J. Easterbrook, C. A. O’Callaghan, A. D. Kelleher, J. A. Whelan, G. Sontag, A. K. Sewell and R. E. Phillips (2000). Highly active antiretroviral therapy for primary HIV-1 infection preserves immune function of virus-specific T lymphocytes. Quarterly Journal of Medicine, 93, 769-70.

36. Sewell, A. K. (2000) Immunity to Human Immunodeficiency virus: Current issues Immunology News, 7.4, 17.

35. Sewell, A. K., and K. Whelan (2000). The role of dendritic cells in HIV-1 infection. Immunology News, 7.4, 22-23.

34. Sewell, A.K., D.A. Price, A. Oxenius, A. D. Kelleher and R. E. Phillips (2000). Cytotoxic T Lymphocyte Responses to HIV: Control and Escape. Stem Cells 18, 230-144.

33. Sewell, A. K. (2000) Does terminal replicating image xeroxing spell the end for monoclonal antibodies? Immunology News 7.2, 35.

32. Sewell, A. K. (2000). HIV-1: Fooling the immune system. Immunology News, 7.2, 31-33.

31. Sewell, A. K.*, C-L. Lin*, G. Gao, K. Whelan, R. E. Philpps and J. A. Austyn (2000). Macrophage tropic HIV induces and exploits dendritic cell chemotaxis. Journal of Experimental Medicine, 192, 587-594.

30. Oxenius, A., D. A. Price, P. J. Easterbrook, C. A. O'Callaghan, J. A. Whelan, A.A. Kellerher, A. K. Sewell and R. E. Phillips (2000). Early highly active antiretroviral therapy for acute HIV-1 infection preserves immune function of CD8+ and CD4+ lymphocytes Proc. Natl. Acad. Sci. USA, 97, 3382-3387.

29. Gileadi, U., H. T. Teisserenc, B. L. Booth, P. R. Dunbar, A. K. Sewell, J. Trowsdale, R. E. Phillips and V. Cerundolo (1999). Generation of an immunodominant cytotoxic T lymphocyte epitope is determined by Proteasome Subunit Composition and Stabipty of the Antigenic Protein. Journal of Immunology, 163, 6045 – 6052.

28. Whelan, J.A., P.R. Dunbar, D.A. Price, M.A. Purbhoo, F. Lechner, G.S. Ogg, G. Griffiths, R.E. Phillips, V. Cerundolo and A.K. Sewell (1999) Specificity of cytotoxic T lymphocyte interactions with peptide-MHC class I tetrameric complexes is temperature dependent. Journal of Immunology, 163, 4342 – 4348.

27. Sewell, A. K., D. A. Price, H. Teisserenc, B. L. Booth, U. Gileadi, R. E. Phillips and V. Cerundolo (1999) IFN- exposes a cryptic cytotoxic T lymphocyte epitopes in HIV 1 reverse transcriptase Journal of Immunology, 162, 7075-9.

26. Sewell, A. K., U. C. Gerth, D. A. Price, M. A. Purbhoo, J. M. Boulter, G. F. Gao, J. I. Bell, R.E. Phillips and B. K. Jakobsen (1999). Antagonism of cytotoxic T lymphocyte activation by soluble CD8. Nature Medicine, 5, 399-404.

25. Price, D. A., P. Klenerman, B. L. Booth, R. E. Phillips and A. K. Sewell (1999). Cytotoxic T lymphocytes, -chemokines and antiviral immunity. Immunology Today, 20, 212-216.

24. Wilson, J. D. K., G.S. Ogg, R. L. Allen, P.J.R. Goulder, A. Kelleher, A. K. Sewell, C. A. O'Callaghan, S. L. Rowland-Jones and A. J. McMichael (1998). Opgoclonal expansions of CD8+ T cells in chronic HIV infection are antigen specific. Journal of Experimental Medicine, 188, 785-790.

23. Lewis, J. W., A. K. Sewell, D. A. Price and T. Elliot (1998). HLA-A*0201 presents TAP-dependent epitopes to CTL in the absence of tapasin. European Journal of Immunology, 28, 3214-3220.

22. Price, D. A., U.C. Meier, P. Klenerman, M.A. Purbhoo, R.E. Phillips and A. K. Sewell (1998). The influence of antigenic variation on cytotoxic T lymphocyte responses in HIV-1 infection. Journal of Molecular Medicine, 76, 699-708.

21. Price D. A., Goulder P. J. R., Klenerman, P., Sewell, A. K., Easterbrook, P. J., Troop, M., Bangham, C. R. M. and Phillips, R. E. (1998) Positive selection mediated by cytotoxic T lymphocytes influences HIV-1 evolution during primary infection. Immunology Letters 56, 438.

20. Price, D.A., A.K. Sewell, T. Dong, R. Tan, P.J.R. Goulder, S.L. Rowland-Jones and R.E. Philpps (1998). Antigen-specific release of -chemokines by anti-HIV-1 cytotoxic T lymphocytes. Current Biology, 8, 355-358.

19. Purhboo, M. A., A. K. Sewell, P. Klenerman, P.J.R. Goulder, B. Jakobsen, K.L. Hilyard, J. I. Bell and R.E. Phillips (1998). Copresentation of natural HIV-1 agonist and antagonist ligands fails to induce the TCR signalling cascade. Proc. Natl. Acad. Sci. USA. 95, 4527-4532.

18. Sewell, A. K., G.C. Harcourt, P.R.J. Goulder, D.A. Price and Phillips, R. E. (1997). Cytotoxic T lymphocyte antagonism by natural peptide ligands requires simultaneous presentation of the agonist and antagonist peptides. European Journal of Immunology, 27, 2323-2329.

17. Goulder, P. J. R., A. K.Sewell, D. Lallo, D.A.Price, J. Whelan, J. Evans, G. Taylor, G. Luzzi, P. Giangrande, R.E. Phillips and A.J. McMichael (1997) Patterns of immunodominance in HIV-1-specific cytotoxic T lymphocyte responses in two HLA-identical siblings with HLA-A*0201 are influenced by epitope mutation. Journal of Experimental Medicine, 185 1423-1433.

16. Price, D. A., P.J.R. Goulder, ., P. Klenerman, A.K. Sewell, P.J. Easterbrook, M. Troop, C.R.M. Bangham and R. E. Phillips (1997). Positive selection of HIV-1 cytotoxic T lymphocyte escape variants during primary infection. Proc. Natl. Acad. Sci. USA 94, 1890-1895.

1991-1995 Postdoc & Faculty, University of Utah

15. Winge, D. R. A.K. Sewell, W. Yu, J.L. Thorvaldsen, F. Yokoya and R. Farrell (1997). "Metal Ion Stress in Yeast" in Metal Ions in Gene Regulation , eds S. Silver and W. Walden, Chapham & Hall, New York. p279-315.

14. Thorvaldsen, J.L., R. Mehra, A.K Sewell, and D.R. Winge (1995). Analysis of copper-induced metallothionein expression using autonomously replicating plasmids in Candida glabrata. Yeast, 11, 1501-1511.

13. Sewell, A.K., F. Yokoya, W. Yu, T. Miyagawa, T. Murayama and D. R. Winge (1995). Mutated yeast heat shock transcription factor exhibits elevated basal transcriptional activation and confers metal resistance. J. Biol. Chem. 270, 25079-25086.

12. Sewell, A.K., Yokoya, F., Miyagawa, T., Murayama, T. and Winge, D. R. (1995). Role of heat shock transcription factor in metal resistance in yeast. J. Cell. Bioc. 21A, 262.

11. Sewell, A.K., L.T. Jensen, J.C. Erickson, R.D. Palmitter and D.R. Winge (1995). Bioactivity of Metallothionein-3 Correlates with its Unique Domain rather than Metal Binding Properties. Biochemistry , 34, p4740-47407.

10. Thorvaldsen, J.L., A.K. Sewell, A. Tanner, J. Peltiers, I.J. Pickering, G. George and Winge, D. R. (1994). Mixed Cu(I), Zn(II) Coordination in the DNA Binding Domain of the AMT1 Transcription Factor from Candida glabrata. Biochemistry, 33, p9566-9577.

9. Yu, W., V. Santhanagopalan, A.K. Sewell, L.T. Jensen and D.R. Winge (1994). "Dominance of Metallothionein in Metal Ion Buffering in Yeast Capable of Synthesis of (EC)nG Isopeptides." Journal of Biological Chemistry, 269, 21010-21015.

8. Erickson, J.C. A.K. Sewell, L. T. Jensen, D.R. Winge and R.D. Palmitter (1994). Enhanced Neurotrophic Activity in Alzheimer's Disease Cortex is not associated with down-regulation metallothionein-III (GIF). Brain Research 649, 297-304. 7. Michelsen, J.W., A.K. Sewell, H.A. Louis, J.I. Olsen, D.R. Davis, D.R. Winge and M.C. Bekerley (1994). Mutation Analysis of the Metal Sites in a LIM domain. J. Biol. Chem. 269, p11108-11113.

6. Macreadie, I.G., A.K. Sewell and D.R. Winge (1994) "The role of metallothionein in Metal Resistance in Yeast" In Metal ions in Fungi eds. G. Winkleman and D.R. Winge, Marcel Dekker. p279-310.

5. Thorvaldsen, J.L., A.K. Sewell, C. McCowen and D.R. Winge (1993) "Regulation of Metallothionein Genes by ACE1 and AMT1 Transcription Factors." J. Biol. Chem. 268, p12512-12518.

PhD. University of Liverpool

4. Tomsett, A.B., A.K. Sewell, S.J Jones, J.R. deMiranda and D.A Thurman (1992) "Metal binding proteins and metal-regulated gene expression in higher plants." In Inducible Plant Proteins, ed. J. L. Wray, Cambridge University Press. p1-24.

3. Tomsett, A.B, A.K. Sewell, S.J. Jones, J.R. deMiranda and D.A Thurman (1991) Metal-regulated gene expression in higher plants. J. Experimental Botany 42, 20-21.

2. Thurman, D.A., D. Salt, A.K. Sewell, M. Thomas, J.R. deMiranda and A.B. Tomsett (1988) Copper inducible Proteins and Genes in Mimulus guttatus. J. Cellular Biochemistry, 12D p342.

1. Salt, D.E., D.A. Thurman, A.B Tomsett and A.K. Sewell (1989) "Copper-inducible phytochelatins of Mimulus guttatus." Proceedings of the Royal Society of London, Series B 236, 79-89.